Professional hunter Theo Bronkhorst and farm owner Honest Ndlovu, are charged with poaching offences for not having the required hunting permit.
US dentist Walter Palmer shot the animal with a crossbow and rifle outside Hwange National Park.
Police say Mr Palmer, who claims he was unaware of the lion’s identity, could also face poaching charges.
The two Zimbabwean men, who accompanied him on the hunt, will appear in court in Victoria Falls, and could face up to 15 years in prison if found guilty.
Mr Palmer says he regrets shooting the well-known animal and believed he was on a legal hunt, saying he relied on professional guides to find a lion and obtain the necessary permits.”
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Source: BBC news